PikoReal Software Release Notes

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PikoReal Software Release Notes Version 2.1 -> 2.2 major changes Relative Quantification 

Corrected calculations to use geometric mean when averaging relative quantities

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Error bars added to relative quantification bars

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Control error bar size (1 – 3 times standard deviation)

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RQ bars sorting option added

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Hide reference genes from RQ graph

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Min RQ and max RQ added to results table (error bar upper and lower values)

High Resolution Melting 

Automatic clustering algorithm added

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Matlab routines installed for the automatic clustering algorithm

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Colors assigned to each cluster automatically

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Control settings for the automatic clustering algorithm

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Distance to cluster average added to the results table

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Ability to force calls to clusters

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Ability to rename clusters

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Default call mode is difference graph instead of scatter plot

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Difference graph is visible without defined standards

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Default temperature range is applied on first analysis

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Scatter plot is detachable

Melt Curve 

Positive derivative calculation added

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On/off control for the slope correction added

Cq determination 

Automatic threshold button changed to tick box

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Automatic threshold calculated automatically with standalone runs imported from USB

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Color calibration values applied immediately post-run when using imported plate layout

Plate layout 

Sample Type colors improved

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Ctrl+z keyboard command activates undo function

Protocol 

Root protocol properties of completed runs are locked

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Time and temperature decrements are handled in the same manner

General 

Replicate column added to results spreadsheets (Cq and Melt)

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Well selector tooltip available at all times

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Default name when saving a new file is current date and time

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Default folder in Settings set to C:\Thermo\PikoReal2\userData

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Shortcut to examples folder added to userData folder

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Improved layout in example files

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Additional example files for HRM and protein melt added

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Updated protocol files

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Manuals added to installation location C:\Thermo\PikoReal2\manuals

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Desktop shortcut to instrument and software manual added during installation

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Graph colors defined by well match RGB color and are not dependent on fluorophore selection

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Protocol log import from USB standalone run supported (requires firmware support)

Known issues remaining 

Exported absolute quantification standard curve equation (Log quantity on Y axis and Cq on X axis) is different from the default analysis equation (Log quantity on X axis and Cq on Y axis)

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Use flip X and Y axis to obtain same equation in the software and the export

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Detached graph may be obscured by main window

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No notification if user tries to save on read only file

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Replicate borders do not disappear when using undo function in plate layout

Version 2.0 -> 2.1 major changes General 

User interphase color scheme changed

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Language support for Japanese and Chinese

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Graphs included in the Excel export

High Resolution Melting 

Completely new analysis module added to the software

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Difference plot analysis

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Scatter plot analysis

Protocol 

Default lid temperature setting changed from automatic to manual 105 degrees Celsius (in the firmware version 2.2.20 automatic settings for lid temperature are too low)

Cq determination 

Default baseline method changed from Curve Minimum to Trend

Version 1.5 -> 2.0 major changes General 

Complete modernization of the user interface

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Virtual Pipetting Tool

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User manual added

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Improved analysis modules functionalities (absolute quantification, melt curve analysis, allelic discrimination, relative quantification)

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Example files added

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Color calibration utility added